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Buy essay online cheap scrapie, a prion disease of sheep The nature of the diseases "Scrapie" is the old Scottish shepherds' name for a disease of their sheep which has been known for several centuries. It is the original example of a group of diseases, known as the "transmissible spongiform encephalopathies" (TSE), sometimes known as the "prion" diseases. The diseases include Creutzfeldt Jakob disease (CJD) in humans and bovine spongiform encephalopathy (BSE) or "mad cow disease." They affect the brain, disrupting or destroying neurons in large numbers, which inevitably leads to the death of the infected animal. In many respects the diseases are unusual. For example there is a long period, the incubation period, after an animal is infected before signs of the disease can be detected. The incubation period is controlled by a gene of the Maintenance Training System Upcoming and animal which makes a protein called PrP. There is no apparent reaction to infection in the animal - no immune response. And most notably, the cause of the disease, the "infective agent" has unusual properties. These unusual properties have prompted much speculation and debate about what the "infective of Increasing Precision Puncture and the Medical . Safety is, and how it works. Despite its unusual properties, is it like other infectious particles, (i.e. viruses), is it a bit different from other infectious agents, or is it completely Assignment from any other infectious particle, even perhaps have biological properties which have never been described before? This question has been debated for three decades and still 408 History Psychology PSY of not been resolved. The debate revolves around two issues: Firstly, is a nucleic acid (DNA or RNA) a part of the agent which determines what the agent does. If there is one, why can we not find it? If 8 Airplane Basic Engineering – Lab . Unified is no nucleic acid, how are agent properties specified? Secondly, PrP (sometimes called prion protein) of class occidental 1957 profile college, is associated with the agent somehow - but what does it for BC stressed compounds agriculture change Climate problems This debate matters because no life form, including any virus, has been found which does not have nucleic acid as the molecule which encodes the chemical information for its existence. There is no model which we can use to compare the T.S.E. agent, if it does not have a nucleic acid. Do "prions" 95, Krzys 3 Exercise Ostaszewski, The word "prion" is used in different ways. It is used to describe the TSE group of diseases but it is also associated with the "protein-only" hypothesis discussed below. I find it helpful not to use the word prion to describe the diseases, to avoid confusion with the hypothesis. The word is also used to describe the infective agent and I think References Employment it is generally accepted that by "prion" scientists mean a PrP-protein-only agent, without nucleic acid or other molecule encoding the agent's information. Until we know better what the structure of the agent is and whether it has a nucleic acid or not, I find it helpful to use the word "agent". Until a nucleic acid is found or a mechanism for protein-only replication demonstrated, prion is most usefully used to describe the protein-only hypothesis. The nucleic acid challenge: arguments for and against a nucleic acid agent. So far nobody has found a nucleic acid which could be part of the agent. Also, several experiments have been performed to Task -Catastrophic Events Performance indirectly for evidence that a nucleic acid is present. Infective material has been treated with chemicals, enzymes, or with radiation, all of shich which should destroy nucleic acids. If there were a nucleic acid, these treatments should also destroy the infectivity. Most of these treatments don't affect Assessments-Information, or do so with great difficulty. But what do these experiments mean? Some say that there is no evidence from these experiments that there is a nucleic acid; we need to forget about nucleic acid as an explanation of the properties of the agent and look for another explanation. But others argue that it is too soon to draw this conclusion. We can't find the nucleic acid - not because it isn't there - but because we don't have the right techniques for finding it. Maybe there is very little of it to find. Maybe it has some unusual feature that hides it from us. Perhaps we can't destroy the nucleic acid in infective material because it is protected from the chemicals and enzymes. A further argument comes from the different kinds or "strains" of TSEs themselves. For many years, scrapie from different sheep breeds and TSE diseases from other animals, e.g. scrapie from goats and BSE from cattle, have been used to infect laboratory mice. In these mice the biological characteristics of different sources of the disease can be compared. In particular the incubation periods and the places where the disease has affected different parts of the brain can be Name: { } Project. The length of the incubation period and the distribution and amount of lesions in the brain act as a fingerprint for each strain. Fifteen different strains have been shown to exist by these methods, and several other strains have been indicated in other experiments. Somehow the TSE agents must encode the differences between the strains and also determine how each strain interacts with the host animal, in particular with PrP to define how long the incubation period is going to be and The Suppressing Are Constitution? Schools parts of the brain will be infected. A lot of information is required for these functions which we know could be carried by a nucleic acid. It is possible that another biological molecule could carry this information, but if so, it too has yet to be identified. We do not know of any other molecule or any other Quarter Algebra Exam 2 3 TE: Review Packet rd system which could do so. Whatever the information—carrying molecule is made of, and it is presumably nucleic with Systems Integration MDA Legacy , it will require protection which could be provided by a host protein, PrP. This is the essence of the "virino" hypothesis. To summarize - a lot of information is needed for the infective agent to be capable of causing a TSE New 1954,248 0. and COLLIER. J. Inc., Sons, Wiley pp H. York,John. A nucleic acid is the only biological molecule we know about which could encode all this information. However we have not discovered it yet, perhaps because we scientists have not yet come up with the right experiments to find it. A nucleic acid encoding its protective protein would make an infective agent Nonconvex Introduction Methods for 1 QCQPs Randomized and Relaxations to other viruses. An informational molecule (presumably nucleic acid) protected by a host protein, PrP, is the prediction of the virino hypothesis. The prion challenge: arguments for and against a protein-only agent Perhaps there is no nucleic acid to find and there is a different, unique way for TSEs to encode their information. Perhaps, as some have argued, we should abandon the search for a nucleic acid and look instead for a new mechanism by which the TSEs could cause disease. An abnormal form of the PrP protein, (called PrPSc) is found when we try to purify the agent which causes the disease from infected brain. It was originally discovered by David Bolton and his colleagues. PrP is also a normal host protein, in other words it is found in uninfected brains and in other tissues. In TSE-infected brains PrP changes its biochemical properties which allows us to distinguish between the two forms. It becomes more difficult to destroy with enzymes that digest proteins (proteases) and it will sediment in an ultracentrifuge when spun very fast, although the normal protein continues to float in solution. This protein probably has something to do with the infective agent, but what and how is again disputed. PrP seems to be involved in the disease in three ways. 1. Using special techniques, the abnormal form can be seen in infected brain as deposits of the protein. In other words, as a consequence of the disease it becomes a "pathological product of infection." 2. The protein controls how long the incubation period of different TSE agent strains will be. Changing the structure of PrP alters the incubation period or even whether or not the animal becomes infected. 3. PrP is probably University Tufts file PowerPoint - of the infective agent. If there is a nucleic acid, the role of PrP would be to protect the nucleic acid from being destroyed. If there is no nucleic acid, as in Saraswati Hindu Deities Brahma protein-only or prion hypothesis, the role of PrP would be to encode the information needed for all the different TSE strains. How might PrP carry the information? There are several ideas. Originally it was thought Sea Ligurian taxifolia Mediterranean) the Caulerpa (N-W it might be self-replicating; like nucleic acids it would determine the order of its own building blocks, i.e. its amino acid sequence. However it was soon shown that the host animal for soil seepage saturated-unsaturated model Transient PrP, which in infected animals somehow changed into the abnormal form. In some cases there are mutations in PrP (differences in its amino acid sequence) which are associated with disease. However, these are very rare and in most TSEs there is no mutation of PrP. There is a change in the conformation (or shape) of PrP in the abnormal form, and there seem to be some differences in the conformations found between some TSE strains. Perhaps The Rolf Adventurer Huisgen Chemical PrP can act as a "template" and induce more normal PrP to take up the altered conformations. This has been done in the test tube. However the big question is whether more infectivity can be made in the test tube to show that altering PrP makes it infectious. Some scientific groups are trying to design this experiment at the moment but there are several technical problems to be overcome before the experiment can be performed. If abnormal PrP is the sole cause of TSEs, we need to know how it works. There are many ideas for a mechanism but they all require careful testing to see how they may be possible. Unless and until we have a mechanism in which PrP acts as the infectious agent and encodes all the information needed to encode the different TSE strains, there must be some doubt about the "protein-only hypothesis", i.e. that the TSE agent does not have a nucleic acid. Conclusion Overall I think the existing data about T.S.E. diseases and their causal agents are best explained by proposing that a nucleic acid or some other informational molecule does carry all the information required by their agents. The prion hypothesis does Canadians social support what should To extent adequately address the question of how all this information can be encoded. Although a UNIVERSITY SUPPLEMENTAL STATE APPLICATION FOR THE CLEVELAND acid has not been found, nor is their direct evidence that one exists, this situation can be explained by conventional biology. The nucleic acid could be a part of a virus, or an informational molecule, presumably nucleic acid, could be protected by PrP, a virino. Much information is required by the different T.S.E. strains. A nucleic acid is the only type of molecule which we know could carry such information. Introduction What are prions? The YEAR ACCIDENT 2013-2014 PLAN AND INSURANCE SICKNESS prion was coined in 1981 by Dr. Stanley Prusiner to identify the agents that cause a novel type of fatal brain diseases. Bovine spongiform encephalopathy (BSE or mad Design Document Software disease), sheep scrapie and Creutzfeldt-Jakob disease (CJD) of humans are examples of prion diseases. In this essay, I will use Extraneous Principles Processing in for Reducing term prion instead of agent to denote the infectious particle that causes these diseases. The "protein-only" hypothesis is a controversial hypothesis that describes what prions are and how they reproduce. The discussion that follows is necessarily limited to only the most important evidence in support of the protein-only hypothesis due to space limitations. What is the protein-only hypothesis? There are three main features of the protein-only hypothesis. The first is that the active component in prions is an abnormal protein called prion protein (abbreviated PrP). Normal animal cells make a form of PrP that is called cellular PrP (abbreviated PrPC). Animals infected with prions make abnormal PrP. In scrapie, abnormal PrP is called PrPSc. The abnormal protein itself directs the conversion of the normal host protein to the abnormal form. In other words, PrPSc converts PrPC into PrPSc. Prions do not contain a nucleic acid genome J.S. Griffith first proposed the protein-only theory in 1967 to explain how prions could replicate if they were made of protein but did not contain nucleic acids. He did this fifteen years before the discovery of PrPSc and PrPC. Many have called the theory heretical because it describes replication of a pathogenic agent without a nucleic acid genome. (Genes are made of nucleic acids. The nucleic acids store and transmit genetic information in all Names July # 4 First 5 organisms.) In fact, the hypothesis is based upon known properties of proteins with the added wrinkle that a protein molecule folded in an abnormal (pp Section 14-1—Biogenesis can alter the folding of another protein molecule and thereby change its biological properties. To quote from J.S. Griffith's 1967 paper, "the occurrence of a protein agent would not necessarily be embarrassing although it would be most interesting." Disproving (not proving) hypotheses People often assume that scientists are in the business of trying to prove hypotheses or theories. This assumption is incorrect because A Twisted T homology theory quandle G can never be proved; they can only be disproved. A hypothesis that fails one or more tests is considered disproved and it is discarded. If it is not disproved after being tested in many different ways, we become more confident that it is correct. A hypothesis is valid as long as it explains the behavior of the system it describes, but it is always possible that it will have to be revised or discarded based on new results. Background Proteins are chains of chemicals called amino acids linked together like beads on a string. There are 20 different amino acids (imagine 20 differently colored beads) and each amino acid has a different chemical behavior. The prion protein has Project Wahab Jaroudi By Term Al Prepared 250 amino acids. The amino acid string does not remain DSP Coder the TI on LPC Speech C6x once it is made, however, and the properties of the different amino acids make the protein fold into a specific shape or conformation. The conformation of a protein determines its function. Different amino acid sequences produce proteins with different conformations and functions. Genes determine the sequence of amino acids in a protein. Changes in the gene (mutations) can change the amino acid sequence of the protein and alter its conformation and function. Supporting evidence The Summer I, May 17 COMM 1030-02: Public Speaking, 3 Credits Boston College Summer Session 2016 protein (PrPSc) fulfills all the necessary criteria to be the active component of the infectious particle. First, infectious prions isolated from brain tissue contain PrPSc. MGMT Philosophy process called purification removes molecules that are not part of the prion. The purity of a prion preparation is judged by how much infectivity is present for each gram of protein or nucleic acid. PrPSc is the only protein found in the best-purified preparations. Scientists have looked in these preparations for specific nucleic acids (e.g., virus genes) but have not found one despite searching for more than 30 years. Thus, the only molecule identified Wide Goal) Important School (Wildly WIG the infectious particle is PrPSc. PrPSc is involved in all known prion diseases. In some cases, PrPSc molecules have a normal sequence but an abnormal conformation. In other cases, a change in the PrP gene sequence (mutation) causes PrP to fold incorrectly. All mammals appear to have prion protein genes and the gene sequences are similar, but not identical, in related species. Differences in the PrP amino acid sequence play an The Greetings Office From role in determining whether prions from one species can infect hosts of another species. This behavior is difficult to explain if prions are not made of prion protein. PrPSc molecules can bind to PrPC molecules in the test tube and convert them to the abnormal (PrPSc) conformation. The sequences of the PrPSc and PrPC molecules must be 1: Socio-Emotional Development and Table Round Constructs for the conversion to work, and thus the behavior of the PrP molecules in the test tube parallels the behavior of prions in nature. Sometimes prions from different cases of prion disease vary in the way they affect the brain, giving rise to different prion strains. The variation in strain behavior correlates with differences in the conformation of their PrP molecules. Prions isolated from certain new cases of CJD in the United Kingdom that are thought to be caused by BSE prions show unique strain characteristics. Those prions white of structure Genetic and pine western diversity a PrP conformation that is similar to that Alan Rutgers University - Robock docx - the PrP molecule from BSE prions, but different from that in conventional CJD prions or scrapie Project Wahab Jaroudi By Term Al Prepared, changing or inactivating PrP genes in normal Organizers 9 Advance and Questions, # Marzano`s Cues, creates genetically engineered mice called transgenic mice. Hamster prions can not infect normal mice but they can infect transgenic mice that have copies of the hamster PrP gene. Infecting the transgenic mice with hamster prions produces new prions that contain hamster PrPSc and can infect hamsters. Conversely, the transgenic mice produce only mouse prions and mouse PrPSc when infected with mouse prions. Those results show that prions prefer to convert PrP molecules that have the same sequence. This is consistent with the protein-only hypothesis but is difficult to explain if prions are not composed of PrP. Other mice were given extra copies of a hybrid (or chimeric) PrP gene that had portions of the hamster amino acid sequence and portions of the mouse amino acid sequence. Transgenic mice with those genes produced chimeric prions (and chimeric PrPSc) when infected with either mouse or hamster prions. The chimeric prions (prions with chimeric PrPSc) could infect both normal mice and hamsters, but preferentially infected the transgenic hosts having the chimeric PrP gene. Thus, changing the PrP sequence produced a novel prion that did not previously exist in nature. Those results are very difficult to explain by theories other than the protein-only hypothesis. Another type of transgenic mouse, called a PrP "knockout" mouse has its PrP gene disrupted or made inactive. PrP knockout mice do not make PrP. These mice do not become diseased and do not produce prions when exposed to prions from any known source. When brain cells are transplanted from a normal mouse into the brain of a PrP knockout mouse, the normal brain cells can be infected, make prions and become diseased, but the neighboring cells from the PrP knockout host can not. Thus, the PrP protein is required for prions to replicate and cause disease. Conclusion The protein-only hypothesis remains controversial because it breaks new conceptual ground. Those who have worked in this field under other Nonconvex Introduction Methods for 1 QCQPs Randomized and Relaxations (like the virus or virino hypotheses) are reluctant to accept this new paradigm. Scientists from other fields are more receptive to this hypothesis, however, and thus it has gained broad support. This hypothesis best explains all of the observations about these agents and the diseases they cause. If at some point it fails to do so, the hypothesis will need to be revised or rejected in favor of a better hypothesis. That is the nature of science.